Understanding Mechanical Emulsification (Nanofat) Versus Enzymatic Isolation of Tissue Stromal Vascular Fraction (tSVF) Cells from Adipose Tissue: Potential Uses in Biocellular Regenerative Medicine

Posted on Posted in Robert W. Alexander, MD, DMD, FICS, Volume 8 : 2016

With rapid growth of experiences and techniques in the field of Biocellular Regenerative Medicine, clinical scientists and biotechnical advancements constantly seek to understand and optimize uses of the multipotent heterogeneous cellular populations found in adipose tissue complex.

Understanding Adipose-derived Stromal Vascular Fraction (AD-SVF) Cell Biology and Use on the Basis of Cellular, Chemical, Structural and Paracrine Components: A Concise Review

Posted on Posted in Robert W. Alexander, MD, DMD, FICS, Volume 4 : 2012

In the past 10 years, biocellular regenerative medicine has recognized the potential value and availability of a heterogeneous, undifferentiated, nucleated cell population with the adipose tissue complex (ATC). With the recognition of higher mesenchymal stem cell concentrations, ease and safety of access, and the very heterogeneous undifferentiated cell populations in the native 3D adipose matrix, many researchers and clinicians are moving from bone marrow sources to the adipose complex.

Stem Cell Prolotherapy in Regenerative Medicine: Background, Theory and Protocols

Posted on Posted in Donna Alderman, DO, Gerald R. Harris, DO, Patrick C. Astourian, MS, PA-C, Robert W. Alexander, MD, DMD, FICS, Volume 3 : Issue 3 : August 2011, Wonder Why?

By Donna D. Alderman, DO, Robert W. Alexander, MD, DMD, FICS, Gerald R. Harris, DO, & Patrick C. Astourian, MS, PA-C ABSTRACT Prolotherapy is a proven technique for resolving musculoskeletal pain, but can have limitations if tissue damage is too severe. Platelet Rich Plasma (PRP) Prolotherapy offers a physiologic tool in some of those cases, but this too may more »

Autologous Fat Grafts as Mesenchymal Stromal Stem Cell Source for use in Prolotherapy: A Simple Technique to Acquire Lipoaspirants

Posted on Posted in Robert W. Alexander, MD, DMD, FICS, Volume 3 : Issue 3 : August 2011, Wonder Why?

ABSTRACT Objectives: Background for use of autologous adipose tissue as a source of adult progenitor (stem) cells for use in Prolotherapy. Present a means of lipoaspiration to harvest adipose-derived mesenchymal stromal cells (AD-MSC) and the stromal vascular fraction (SVF) for use in Prolotherapy and regenerative medical applications by non-plastic surgeons. Design: Explain the patented super luer-lok and more »